A farnesylated Coxiella burnetii effector forms a multimeric complex at the mitochondrial outer membrane during infection

LF Fielden; JH Moffatt; Y Kang; MJ Baker; CA Khoo; CR Roy; D Stojanovski; HJ Newton

Journal article

A farnesylated Coxiella burnetii effector forms a multimeric complex at the mitochondrial outer membrane during infection

LF Fielden, JH Moffatt, Y Kang, MJ Baker, CA Khoo, CR Roy, D Stojanovski, HJ Newton

Infection and Immunity | AMER SOC MICROBIOLOGY | Published : 2017

DOI: 10.1128/IAI.01046-16

Abstract

Coxiella burnetii, the causative agent of Q fever, establishes a unique lysosome-derived intracellular niche termed the Coxiella-containing vacuole (CCV). The Dot/Icm-type IVB secretion system is essential for the biogenesis of the CCV and the intracellular replication of Coxiella. Effector proteins, translocated into the host cell through this apparatus, act to modulate host trafficking and signaling processes to facilitate CCV development. Here we investigated the role of CBU0077, a conserved Coxiella effector that had previously been observed to localize to lysosomal membranes. CBU0077 was dispensable for the intracellular replication of Coxiella in HeLa and THP-1 cells and did not appear..

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University of Melbourne Researchers

Yilin Kang Author

Diana Stojanovski Author

Hayley Newton Author

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HOW DO Q FEVER CAUSING BACTERIA MANIPULATE THE HUMAN HOST TO CAUSE DISEASE?

This study explores how the bacterium Coxiella burnetii causes the serious infectious disease Q fever. Coxiella is a potential biological we..

HOW DO Q FEVER CAUSING BACTERIA GROW INSIDE HUMAN CELLS?

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Grants

Awarded by National Institute of Allergy and Infectious Diseases

Funding Acknowledgements

This research was supported by project grants awarded to H.J.N. from the Australian National Health and Medical Research Council (grant numbers 1062383 and 1063646). D.S. is supported by a Biochemistry Fund fellowship through the Department of Biochemistry and Molecular Biology, The University of Melbourne. L.F.F. is supported by a postgraduate research scholarship through the Department of Biochemistry and Molecular Biology, The University of Melbourne. Y.K. is supported by a Melbourne International Research Scholarship and Melbourne International Fee Remission Scholarship.